Abstract
A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (approximately 7 Mohms) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosensing Techniques / instrumentation*
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Biosensing Techniques / methods
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Capsaicin / pharmacology
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Cell Line
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Humans
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Ion Channels / chemistry*
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Ion Channels / drug effects
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Ion Channels / metabolism
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Kidney / cytology
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Kidney / drug effects
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Kidney / metabolism
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Ligands
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Patch-Clamp Techniques / instrumentation
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Patch-Clamp Techniques / methods
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Silicon / chemistry*
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TRPV Cation Channels / chemistry
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TRPV Cation Channels / drug effects
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TRPV Cation Channels / metabolism
Substances
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Ion Channels
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Ligands
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TRPV Cation Channels
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TRPV1 receptor
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Capsaicin
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Silicon