Crystallization of an Lhx3-Isl1 complex

Mugdha Bhati; Mihwa Lee; Amy Louise Nancarrow; Ingolf Bach; J Mitchell Guss; Jacqueline Mary Matthews

doi:10.1107/S174430910800691X

Crystallization of an Lhx3-Isl1 complex

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Apr 1;64(Pt 4):297-9. doi: 10.1107/S174430910800691X. Epub 2008 Mar 21.

Authors

Mugdha Bhati¹, Mihwa Lee, Amy Louise Nancarrow, Ingolf Bach, J Mitchell Guss, Jacqueline Mary Matthews

Affiliation

¹ School of Molecular and Microbial Biosciences, The University of Sydney, Australia.

Abstract

A stable intramolecular complex comprising the LIM domains of the LIM-homeodomain protein Lhx3 tethered to a peptide region of Isl1 has been engineered, purified and crystallized. The monoclinic crystals belong to space group C2, with unit-cell parameters a = 119, b = 62.2, c = 51.9 A, beta = 91.6 degrees , and diffract to 2.05 A resolution.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
Genetic Vectors / chemical synthesis
Homeodomain Proteins / biosynthesis
Homeodomain Proteins / chemical synthesis
Homeodomain Proteins / chemistry*
Homeodomain Proteins / genetics
LIM-Homeodomain Proteins
Protein Engineering
Transcription Factors
Zinc Fingers / genetics

Substances

Homeodomain Proteins
LIM-Homeodomain Proteins
Lhx3 protein
Transcription Factors