Site-specific immobilization of CMP-sialic acid synthetase on magnetic nanoparticles and its use in the synthesis of CMP-sialic acid

Ching-Ching Yu; Po-Chiao Lin; Chun-Cheng Lin

doi:10.1039/b716330d

Site-specific immobilization of CMP-sialic acid synthetase on magnetic nanoparticles and its use in the synthesis of CMP-sialic acid

Chem Commun (Camb). 2008 Mar 21:(11):1308-10. doi: 10.1039/b716330d. Epub 2008 Jan 23.

Authors

Ching-Ching Yu¹, Po-Chiao Lin, Chun-Cheng Lin

Affiliation

¹ Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.

PMID: 18389116
DOI: 10.1039/b716330d

Abstract

Through the native chemical ligation, CMP-sialic acid synthetase (CSS) was site-specifically immobilized on magnetic nanoparticles and presented excellent enzymatic performance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Cytidine Monophosphate N-Acetylneuraminic Acid / chemical synthesis*
Dithiothreitol
Electrophoresis, Polyacrylamide Gel
Enzymes, Immobilized / chemistry*
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / genetics
Indicators and Reagents
Models, Molecular
N-Acylneuraminate Cytidylyltransferase / chemistry*
Nanoparticles
Neisseria meningitidis, Serogroup B / enzymology

Substances

Enzymes, Immobilized
Indicators and Reagents
Green Fluorescent Proteins
Cytidine Monophosphate N-Acetylneuraminic Acid
N-Acylneuraminate Cytidylyltransferase
Dithiothreitol