The main aim of the study was to investigate the changes of hematologic status in elite soccer players throughout a competitive season. Study was conducted with 35 male professional soccer players and the measurements were collected at the start of the conditioning period, at the start of the season, in the mid-season and at the end of the season. Blood was drawn from an antecubital vein for a complete blood count, serum iron, and transferrin and ferritin levels. We found significantly higher hematocrit at preseason assessment as compared with other sampling periods (P < 0.05). No other differences were found between any of the hematologic variables during the study. The lowest hemoglobin level, mean corpuscular volume (MCV), serum iron, ferritin and transferrin encountered in the study were Hb of 11.2 g/dl, MCV of 77.9 fl, serum iron of 34 microg/dl, ferritin of 15.1 microg/l, transferrin of 224 microg/dl in a 20-year-old soccer player at the start of the season. The variability of the iron status indicators in elite soccer players seems to be stable and poorly related to training phase during sports season.