Blue native electrophoresis study on lipases

M Saminathan; K Thirumalai Muthukumaresan; Srinivas Rengarajan; Nandhini Muthukrishnan; Pennathur Gautam

doi:10.1016/j.ab.2008.03.010

Blue native electrophoresis study on lipases

Anal Biochem. 2008 Jun 15;377(2):270-1. doi: 10.1016/j.ab.2008.03.010. Epub 2008 Mar 14.

Authors

M Saminathan¹, K Thirumalai Muthukumaresan, Srinivas Rengarajan, Nandhini Muthukrishnan, Pennathur Gautam

Affiliation

¹ Centre for Biotechnology, Anna University, Chennai 600025, India.

PMID: 18381198
DOI: 10.1016/j.ab.2008.03.010

Abstract

We have developed a modified blue native polyacrylamide gel electrophoresis (PAGE) protocol that can overcome aggregation of lipases seen in native PAGE. We have shown that two lipases, Pseudomonas aeruginosa lipase and Candida rugosa lipase, which aggregate in the native gel, can be resolved using our protocol. Activity staining was done to test for the functionality of the two lipases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Candida / enzymology
Electrophoresis, Polyacrylamide Gel / methods*
Lipase / metabolism*
Pseudomonas aeruginosa / enzymology
Staining and Labeling

Substances

Lipase