Abstract
Targeted antisense and gene replacement mutagenesis experiments demonstrate that only the murA1 gene and not the murA2 gene is required for the normal cellular growth of Bacillus anthracis. Antisense-based modulation of murA1 gene expression hypersensitizes cells to the MurA-specific antibiotic fosfomycin despite the normally high resistance of B. anthracis to this drug.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
MeSH terms
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Alkyl and Aryl Transferases / genetics*
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Alkyl and Aryl Transferases / metabolism*
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Anti-Bacterial Agents / pharmacology
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Bacillus anthracis / drug effects
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Bacillus anthracis / enzymology*
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Bacillus anthracis / genetics
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Bacillus anthracis / growth & development*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Drug Resistance, Bacterial
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Fosfomycin / pharmacology
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Inhibitory Concentration 50
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Microbial Sensitivity Tests
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RNA, Antisense / genetics
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RNA, Antisense / metabolism
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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RNA, Antisense
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Fosfomycin
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Alkyl and Aryl Transferases
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UDP-N-acetylglucosamine 1-carboxyvinyltransferase