The dynamics of industrial types of glycerol as a supplementary carbon source to glucose for beta-carotene production by Blakeslea trispora was investigated in batch cultures. The growth kinetics, cellular lipid accumulation-degradation, substrate assimilation, and beta-carotene production were clearly dependent on the level of addition of pure glycerol. The highest beta-carotene production (15.0 mg/g of dry biomass) was obtained at an initial glycerol concentration of 60.0 g/L. Substitution of pure glycerol by the nonpurified soap byproduct did not inhibit cell growth. Conversely, partial purification of the biodiesel byproduct by removing methanol and fatty acids was unavoidable for cell growth. Both types of industrial glycerol stimulated beta-carotene synthesis more than 10 (soap byproduct) and 8 times (biodiesel byproduct) compared to control medium. The maximum beta-carotene contents were 10 and 8 mg/g of dry biomass, respectively, and its relative content in the carotenoid fraction was 86-88%.