Using ninhydrin to detect gravesoil

J Forensic Sci. 2008 Mar;53(2):397-400. doi: 10.1111/j.1556-4029.2008.00681.x.

Abstract

Some death scene investigations commence without knowledge of the location of the body and/or decomposition site. In these cases, it is necessary to locate the remains or the site where the body decomposed prior to movement. We hypothesized that the burial of a mammalian cadaver will result in the release of ninhydrin reactive nitrogen (NRN) into associated soil and that this reaction might have potential as a tool for the identification of clandestine graves. Juvenile rat (Rattus rattus) cadavers were buried in three contrasting soil types in Australian tropical savanna ecosystems and allowed to decompose over a period of 28 days. Soils were sequentially harvested and analyzed for NRN. Cadaver burial resulted in an approximate doubling (mean = 1.7 +/- 0.1) in the concentration of soil NRN. This reaction has great potential to be used as a presumptive test for gravesoil and this use might be greatly enhanced following more detailed research.

MeSH terms

  • Animals
  • Burial*
  • Forensic Anthropology
  • Indicators and Reagents
  • Models, Animal
  • Ninhydrin*
  • Nitrogen / analysis
  • Postmortem Changes*
  • Rats
  • Soil / analysis*
  • Time Factors

Substances

  • Indicators and Reagents
  • Soil
  • Ninhydrin
  • Nitrogen