Abstract
Anti-HAV IgM positive serum samples from acute phase hepatitis A patients from various areas in Turkey were tested for viral RNA by RT-PCR (reverse transcriptase polymerase chain reaction), using primer pairs from two different regions of the HAV genome. The PCR products amplified from both genomic regions underwent phylogenetic analyses. A comparison of the regions showed the same genotyping results, and the RT-PCR-2 in the 5'NCR demonstrated greater sensitivity compared to RT-PCR-1 in the VP1-P2A region. The majority of the isolates belonged to genotype IB and are related closely to each other; however, two isolates related even more strongly to the HAV HM175 strain. Two (n = 37) RT-PCR positive sera were classified under genotype IA. A surprising finding emerged for the mean levels of serum transaminases AST and ALT: higher levels were found in patients under 10 years of age compared to older patients. Anti-HAV IgM levels were determined quantitatively and, in addition, the HAV-RNA genome equivalents were ascertained by real time RT-PCR. No evidence was found for an association between viral load and the higher transaminase levels in the younger group.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Untranslated Regions / genetics
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Adolescent
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Adult
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Age Factors
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Alanine Transaminase / blood
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Aspartate Aminotransferases / blood
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Child
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Child, Preschool
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Female
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Genotype
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Hepatitis A / epidemiology
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Hepatitis A / virology*
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Hepatitis A Antibodies / blood
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Hepatitis A virus / classification*
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Hepatitis A virus / genetics*
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Hepatitis A virus / isolation & purification
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Humans
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Immunoglobulin M / blood
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Male
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Middle Aged
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Molecular Sequence Data
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Phylogeny
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RNA, Viral / blood
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology
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Turkey / epidemiology
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Viral Structural Proteins / genetics
Substances
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5' Untranslated Regions
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Hepatitis A Antibodies
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Immunoglobulin M
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RNA, Viral
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VP1 protein, hepatitis A virus
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Viral Structural Proteins
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Aspartate Aminotransferases
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Alanine Transaminase
Associated data
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GENBANK/AM910873
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GENBANK/AM910874
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GENBANK/AM910875
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GENBANK/AM910876
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GENBANK/AM910877
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GENBANK/AM910878
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GENBANK/AM910879
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GENBANK/AM910880
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GENBANK/AM910881
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GENBANK/AM910882
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GENBANK/AM910883
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GENBANK/AM910884
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GENBANK/AM910885
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GENBANK/AM910886
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GENBANK/AM910887
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GENBANK/AM910888
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GENBANK/AM910889
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GENBANK/AM910890
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GENBANK/AM910891
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GENBANK/AM910892
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GENBANK/AM910893
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GENBANK/AM910894
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GENBANK/AM910895
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GENBANK/AM910896
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GENBANK/AM910897
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GENBANK/AM910898
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GENBANK/AM910899
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GENBANK/AM910900
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GENBANK/AM910901
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GENBANK/AM910902
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GENBANK/AM910903
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GENBANK/AM910906
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GENBANK/AM910907
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GENBANK/AM910908
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GENBANK/AM910909
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GENBANK/AM910910
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GENBANK/AM910911
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GENBANK/AM910913
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GENBANK/AM910915
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GENBANK/AM910916
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GENBANK/AM910919
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GENBANK/AM910920
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GENBANK/AM910921
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GENBANK/AM910922
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GENBANK/AM910923
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GENBANK/AM910924