Purpose: To evaluate the effect of overdose fluoride on the expression of Cbfalpha1 in the ameloblasts of rat incisor and its correlation with dental fluorosis at protein level.
Methods: Twenty Wistar rats were divided randomly into two groups, one was fed with distilled water(control group), the other was fed with distilled water of 100ppm F-(experimental group). The incisors of these rats were extracted for immunohistochemical staining eight weeks later to investigate the expression of Cbfalpha1.The data was analyzed with Axioplan 2 imaging system and SPSS10.0 software package.
Results: Cbfalpha1 was expressed in the nuclear of ameloblasts at secretory stage, and the expression level in the experimental group was significantly higher than that in the control group(P<0.01).
Conclusion: Overdose fluoride causes higher expression of Cbfalpha1 in the ameloblasts at secretory stage,which may effect the mineralization and development of enamel in a certain way and leads to the abnormal development of enamel.