Amplification by long RT-PCR of near full-length norovirus genomes

J Virol Methods. 2008 May;149(2):226-30. doi: 10.1016/j.jviromet.2008.02.001. Epub 2008 Mar 19.

Abstract

A long RT-PCR method was developed to amplify the norovirus genome. Starting from RNA extracted directly from clinical samples and using broadly reactive primers, it can generate near full-length amplicons that allow for easy determination of the near complete genomic sequence. Two norovirus isolates from Toronto, Canada, in 2002 and 2005 were sequenced. This approach will facilitate molecular epidemiology studies of noroviruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caliciviridae Infections / virology
  • Canada
  • Feces / virology
  • Genome, Viral*
  • Humans
  • Molecular Epidemiology / methods
  • Molecular Sequence Data
  • Norovirus / classification
  • Norovirus / genetics*
  • Norovirus / isolation & purification
  • Phylogeny
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • RNA, Viral

Associated data

  • GENBANK/EF202567
  • GENBANK/EF202568