Evaluation of restriction enzymes for standardizing pulsed-field gel electrophoresis protocol for rapid subtyping of Vibrio parahaemolyticus

You-Wun Wang; Lih-Ling Chern; Phung Dac Cam; Chien-Shun Chiou

doi:10.1016/j.diagmicrobio.2008.01.024

Evaluation of restriction enzymes for standardizing pulsed-field gel electrophoresis protocol for rapid subtyping of Vibrio parahaemolyticus

Diagn Microbiol Infect Dis. 2008 Jul;61(3):251-5. doi: 10.1016/j.diagmicrobio.2008.01.024. Epub 2008 Mar 14.

Authors

You-Wun Wang¹, Lih-Ling Chern, Phung Dac Cam, Chien-Shun Chiou

Affiliation

¹ The Central Region Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taichung City 40855, Taiwan.

PMID: 18343074
DOI: 10.1016/j.diagmicrobio.2008.01.024

Abstract

We evaluated the cost-effectiveness of the restriction enzymes with rare-cutting sites in the genome of Vibrio parahaemolyticus RIMD 2210633 for pulsed-field gel electrophoresis (PFGE) analysis. The evaluation indicated that PFGE with both NotI and SfiI was discriminatory, but NotI was more cost-effective. Based on the results of this study, we suggest using NotI and SfiI as the 1st and the 2nd restriction enzyme for standardizing the PulseNet PFGE protocol for molecular subtyping and global surveillance of V. parahaemolyticus.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods*
DNA Restriction Enzymes* / economics
DNA, Bacterial / genetics
Electrophoresis, Gel, Pulsed-Field / economics
Electrophoresis, Gel, Pulsed-Field / standards*
Genotype
Vibrio parahaemolyticus / classification*
Vibrio parahaemolyticus / genetics

Substances

DNA, Bacterial
DNA Restriction Enzymes