High-fat diet may impair K(ATP) channels in vascular smooth muscle cells

Biomed Pharmacother. 2009 Feb;63(2):165-70. doi: 10.1016/j.biopha.2008.01.005. Epub 2008 Feb 20.

Abstract

K(ATP) channel in vascular smooth muscle cell (VSMC) is closely linked to the etiology of hypertension. The aim of this study was to investigate effect of the high-fat diet-induced obesity on K(ATP) channel and blood pressure. Obesity was induced by a 24-week high-fat diet feeding in rats. Function and expression of K(ATP) channel in mesenteric arteries were examined using myography system, patch clamp and Western blotting. We show that high-fat diet increased blood pressure, decreased K(ATP) channel-mediated relaxation responses and currents, and down-regulated K(ATP) expression in VSMC. In conclusion, diet-induced obesity impairs K(ATP) channels in VSMC, which may underscore obesity-triggered increase in blood pressure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Pressure
  • Blotting, Western
  • Dietary Fats / adverse effects*
  • Disease Models, Animal
  • Down-Regulation
  • Hypertension / etiology
  • KATP Channels / metabolism*
  • Male
  • Mesenteric Arteries / metabolism
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / metabolism*
  • Myocytes, Smooth Muscle / metabolism
  • Myography
  • Obesity / etiology
  • Obesity / physiopathology*
  • Patch-Clamp Techniques
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Dietary Fats
  • KATP Channels