Molecular imprinting is a useful technique for the preparation of functional materials with molecular recognition properties. In this work, a biomimetic potentiometric sensor, based on a non-covalent imprinted polymer, was fabricated for the recognition and determination of hydroxyzine in tablets and biological fluids. The molecularly imprinted polymer (MIP) was synthesized by precipitation polymerization, using hydroxyzine dihydrochloride as a template molecule, methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylat (EGDMA) as a cross-linking agent. The sensor showed a high selectivity and a sensitive response to the template in aqueous system. The MIP-modified electrode exhibited a Nernstian response (29.4+/-1.0 mV decade(-1)) in a wide concentration range of 1.0x10(-6) to 1.0x10(-1) M with a lower detection limit of 7.0x10(-7) M. The electrode demonstrated a response time of approximately 15s, a high performance and a satisfactory long-term stability (more than 5 months). The method has the requisite accuracy, sensitivity and precision to assay hydroxyzine in tablets and biological fluids.