Structure of human argininosuccinate synthetase

Acta Crystallogr D Biol Crystallogr. 2008 Mar;64(Pt 3):279-86. doi: 10.1107/S0907444907067455. Epub 2008 Feb 20.

Abstract

Argininosuccinate synthetase catalyzes the transformation of citrulline and aspartate into argininosuccinate and pyrophosphate using the hydrolysis of ATP to AMP and pyrophosphate. This enzymatic process constitutes the rate-limiting step in both the urea and arginine-citrulline cycles. Previous studies have investigated the crystal structures of argininosuccinate synthetase from bacterial species. In this work, the first crystal structure of human argininosuccinate synthetase in complex with the substrates citrulline and aspartate is presented. The human enzyme is compared with structures of argininosuccinate synthetase from bacteria. In addition, the structure also provides new insights into the function of the numerous clinical mutations identified in patients with type I citrullinaemia (also known as classic citrullinaemia).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / chemistry
  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Argininosuccinate Synthase / chemistry*
  • Argininosuccinate Synthase / genetics
  • Binding Sites
  • Crystallography, X-Ray
  • Humans
  • Models, Molecular*
  • Molecular Sequence Data
  • Protein Conformation
  • Sequence Analysis, Protein
  • Substrate Specificity

Substances

  • Adenosine Triphosphate
  • Argininosuccinate Synthase