Dramatic improvement in FMD DNA vaccine efficacy and cross-serotype antibody induction in pigs following a protein boost

Vaccine. 2008 May 19;26(21):2647-56. doi: 10.1016/j.vaccine.2008.01.037. Epub 2008 Feb 12.

Abstract

To overcome the low and slow development of humoral antibody often observed with DNA vaccines we applied a prime-boost strategy. When FMD DNA vaccine P1-2A3C3D and pGM-CSF primed pigs were boosted with inactivated foot-and-mouth disease virus (FMDV) antigen and recombinant 3D (without adjuvant) an average 36-fold increase in the FMDV antibody response was observed compared to conventional vaccination, that included a log(10) virus neutralising titre increase. Most remarkably, a significant level of cross-serotype reactivity was observed against A, C and Asia1 in the virus neutralisation and ELISA tests. This prime-boost strategy fully protected pigs from a heterologous challenge.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Antigens, Viral / immunology
  • Cross Reactions
  • Foot-and-Mouth Disease / immunology
  • Foot-and-Mouth Disease / prevention & control*
  • Foot-and-Mouth Disease Virus / immunology*
  • Immunization, Secondary / methods*
  • Neutralization Tests
  • Recombinant Proteins / immunology
  • Swine
  • Vaccines, DNA / immunology*
  • Vaccines, Inactivated / immunology
  • Viral Nonstructural Proteins / immunology
  • Viral Proteins / immunology*
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Recombinant Proteins
  • Vaccines, DNA
  • Vaccines, Inactivated
  • Viral Nonstructural Proteins
  • Viral Proteins
  • Viral Vaccines
  • virus-infection-associated antigen, Foot-and-mouth disease virus