Abstract
To overcome the low and slow development of humoral antibody often observed with DNA vaccines we applied a prime-boost strategy. When FMD DNA vaccine P1-2A3C3D and pGM-CSF primed pigs were boosted with inactivated foot-and-mouth disease virus (FMDV) antigen and recombinant 3D (without adjuvant) an average 36-fold increase in the FMDV antibody response was observed compared to conventional vaccination, that included a log(10) virus neutralising titre increase. Most remarkably, a significant level of cross-serotype reactivity was observed against A, C and Asia1 in the virus neutralisation and ELISA tests. This prime-boost strategy fully protected pigs from a heterologous challenge.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood
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Antigens, Viral / immunology
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Cross Reactions
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Foot-and-Mouth Disease / immunology
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Foot-and-Mouth Disease / prevention & control*
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Foot-and-Mouth Disease Virus / immunology*
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Immunization, Secondary / methods*
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Neutralization Tests
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Recombinant Proteins / immunology
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Swine
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Vaccines, DNA / immunology*
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Vaccines, Inactivated / immunology
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Viral Nonstructural Proteins / immunology
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Viral Proteins / immunology*
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Viral Vaccines / immunology*
Substances
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Antibodies, Viral
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Antigens, Viral
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Recombinant Proteins
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Vaccines, DNA
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Vaccines, Inactivated
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Viral Nonstructural Proteins
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Viral Proteins
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Viral Vaccines
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virus-infection-associated antigen, Foot-and-mouth disease virus