Signal amplification via intramolecular energy transfer using tripodal neutral iridium(III) complexes upon binding to avidin

Tae-Hyuk Kwon; Jongchul Kwon; Jong-In Hong

doi:10.1021/ja710803p

Signal amplification via intramolecular energy transfer using tripodal neutral iridium(III) complexes upon binding to avidin

J Am Chem Soc. 2008 Mar 26;130(12):3726-7. doi: 10.1021/ja710803p. Epub 2008 Mar 4.

Authors

Tae-Hyuk Kwon¹, Jongchul Kwon, Jong-In Hong

Affiliation

¹ Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.

PMID: 18314987
DOI: 10.1021/ja710803p

Abstract

A neutral tripod system for biotin-avidin assays, which is composed of biotin, FIrpic as an energy acceptor, and mCP as an energy donor, offers remarkable sensitivity over traditional transition metal based protein probes due to the intramolecular energy transfer and increased hydrophobicity associated with the avidin binding site and neutral probe 1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Avidin / chemistry*
Binding Sites
Biotin / chemistry
Energy Transfer
Hydrophobic and Hydrophilic Interactions
Iridium / chemistry*
Luminescence
Molecular Structure
Organometallic Compounds / chemical synthesis*
Organometallic Compounds / chemistry
Sensitivity and Specificity

Substances

Organometallic Compounds
Avidin
Iridium
Biotin