The aim of the study was to develop and validate a specific semi-quantitative food frequency questionnaire to assess nutritional intake of Greek adolescents. The sample of the study consisted of 250 pupils (15.3 +/- 0.7 years), who completed the Youth Adolescent Food Frequency Questionnaire enriched with 22 Greek foods and recipes to include ethnic and racial diversity. A 3-day weighed food recall was used as the criterion to test the validity of the questionnaire. The analysis of correlation revealed significant correlations between the two methods for almost all variables. The Pearson's coefficients ranged from 0.83 for energy intake to 0.34 for folate intake. Non-significant correlations were found for selenium and vitamin D intakes. The findings of the study provide evidence for the validity of the scale and its utility in assessing nutritional intake of Greek adolescents.