Objective: To study the chemical constituents of the whote plant Biebersteinia heterostemon (Geraniaceae).
Method: The ethanol extract of the whole plants was separated by various chromatographic methods and the compounds from the extract were identified by spectroscopic evidence including MS, IR, NMR and X-ray crystallographic analysis.
Result: Three isoprenyl guanidine derivatives were isolated from the whole plant of Biebersteinia heterostemon and identified as galegine (1) , cis-4-hydroxygalegine (2) and trans-4-hydroxygalegine (3).
Conclusion: The three compounds were isolated from this plant for the first time.