The use of air sampling devices to measure the concentrations of airborne bacteria in clinical investigations and research trials in calf barns has indicated that traditional systems of ventilation are problematic in cold weather. Individual pen designs should have two solid sides, but the front and rear should be as open as possible. Thermal stress should be managed by providing deep bedding and not by enclosing the pen. Air hygiene can be improved by reducing stocking density and using supplemental positive-pressure ventilation systems to deliver small amounts of air to each pen. Implementation of these recommendations can produce calf barns that seem to equal calf hutches in minimizing disease and provide better working conditions for the caregivers.