Expression of alternative oxidase gene (aox1) at the stage of single-cell conidium in citric acid-producing Aspergillus niger

Takasumi Hattori; Yuki Honda; Kuniki Kino; Kohtaro Kirimura

doi:10.1263/jbb.105.55

Expression of alternative oxidase gene (aox1) at the stage of single-cell conidium in citric acid-producing Aspergillus niger

J Biosci Bioeng. 2008 Jan;105(1):55-7. doi: 10.1263/jbb.105.55.

Authors

Takasumi Hattori¹, Yuki Honda, Kuniki Kino, Kohtaro Kirimura

Affiliation

¹ Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo, Japan.

PMID: 18295720
DOI: 10.1263/jbb.105.55

Abstract

Mycelia of citric acid-producing Aspergillus niger WU-2223L show cyanide-insensitive respiration catalyzed by alternative oxidase. In this study, the constitutive expression of alternative oxidase gene (aox1) even at the stage of single-cell conidium in A. niger WU-2223L was found using the visual expression analysis system of aox1 with green fluorescent protein under microscopy observation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus niger / enzymology
Aspergillus niger / genetics
Aspergillus niger / physiology*
Citric Acid / metabolism*
Gene Expression*
Genes, Fungal*
Green Fluorescent Proteins / analysis
Green Fluorescent Proteins / genetics
Mitochondrial Proteins
Oxidoreductases / genetics*
Plant Proteins
Spores, Fungal / enzymology
Spores, Fungal / genetics
Spores, Fungal / growth & development

Substances

Mitochondrial Proteins
Plant Proteins
Green Fluorescent Proteins
Citric Acid
Oxidoreductases
alternative oxidase