Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants

Org Biomol Chem. 2008 Mar 7;6(5):851-3. doi: 10.1039/b719288f. Epub 2008 Feb 4.

Abstract

The substrate binding regions of a beta-1,3:1,4 glucanase are revealed through structural analysis with a thio-oligosaccharide and kinetics of enzyme variants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Carbohydrate Conformation
  • Crystallography, X-Ray
  • Glycoside Hydrolases / chemistry*
  • Kinetics
  • Models, Molecular
  • Oligosaccharides / chemistry*
  • Protein Conformation
  • Protein Structure, Tertiary
  • Sulfhydryl Compounds / chemistry*

Substances

  • Oligosaccharides
  • Sulfhydryl Compounds
  • Glycoside Hydrolases
  • licheninase