Abstract
We used a complete spinal cord transection model in which the T8 spinal segment was removed to study the effect of combined treatment of peripheral nerve graft and application of FGF-1 on the glial environment. The combined treatment resulted in reduced astrocytic glial scarring, reactive macrophage gliosis, and inhibitory proteoglycan in the back-degenerated white matter tract. While the macrophage activities in the back-degenerative tract were down-regulated, those in the grafted peripheral nerves and in the distal Wallerian degenerative tracts were not. We concluded that the combined treatment changed the glial environment in the back-degenerative tract, and differentially regulated the macrophage activities in the system, in favor of CNS regeneration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Astrocytes / drug effects
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Astrocytes / physiology
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Biomarkers / analysis
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Biomarkers / metabolism
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Disease Models, Animal
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Down-Regulation / drug effects
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Down-Regulation / physiology
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Female
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Fibroblast Growth Factor 1 / pharmacology*
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Fibroblast Growth Factor 1 / therapeutic use
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Glial Fibrillary Acidic Protein / metabolism
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Gliosis / etiology
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Gliosis / physiopathology
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Gliosis / prevention & control*
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Graft Survival / drug effects
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Graft Survival / physiology
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Macrophages / drug effects
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Macrophages / physiology
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Nerve Regeneration / drug effects
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Nerve Regeneration / physiology
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Neural Pathways / drug effects
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Neural Pathways / pathology
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Neural Pathways / physiopathology
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Neuroglia / drug effects
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Neuroglia / physiology
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Peripheral Nerves / cytology
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Peripheral Nerves / physiology
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Peripheral Nerves / transplantation*
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Proteoglycans / metabolism
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Rats
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Rats, Sprague-Dawley
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Spinal Cord Injuries / drug therapy*
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Spinal Cord Injuries / physiopathology
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Spinal Cord Injuries / surgery*
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Tissue Transplantation / methods*
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Treatment Outcome
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Wallerian Degeneration / etiology
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Wallerian Degeneration / physiopathology
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Wallerian Degeneration / prevention & control
Substances
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Biomarkers
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Glial Fibrillary Acidic Protein
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Proteoglycans
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Fibroblast Growth Factor 1