In silico modulation of HMGN-1 binding to histones and gene expression by interplay of phosphorylation and O-GlcNAc modification

Protein Pept Lett. 2008;15(2):193-9. doi: 10.2174/092986608783489607.

Abstract

Utilizing different computational methods; phosphorylation, O-GlcNAc modification and Yin Yang sites are predicted in HMGN-1. Prediction results suggest that interplay of phosphorylation and O-GlcNAc modification regulates binding and removal of HMGN-1 with the nucleosome and its translocation from nucleus to cytoplasm and back to nucleus, consequently modulating gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / chemistry*
  • Acetylglucosamine / metabolism
  • Active Transport, Cell Nucleus / physiology
  • Amino Acid Sequence
  • Animals
  • Cell Nucleus / metabolism
  • Computer Simulation
  • Cytoplasm / metabolism
  • Gene Expression Regulation*
  • HMGN1 Protein* / chemistry
  • HMGN1 Protein* / genetics
  • HMGN1 Protein* / metabolism
  • Histones / metabolism*
  • Humans
  • Molecular Sequence Data
  • Nucleosomes / metabolism
  • Phosphorylation
  • Protein Binding
  • Protein Processing, Post-Translational*
  • Sequence Alignment

Substances

  • HMGN1 Protein
  • Histones
  • Nucleosomes
  • Acetylglucosamine