XopN was originally identified from Xanthomonas campestris pv. vesicatoria as an effector translocated into plant cells via the type III secretion system (T3SS), and is required for pathogenicity. We report here that the xopN homologue in the X. campestris pv. campestris genome, named xopXccN, also encodes a T3SS effector and is required for full virulence. We further demonstrate that expression of xopXccN is positively regulated by the key hrp (hypersensitive response and pathogenicity) regulators HrpG and HrpX.