Nuclease tolerant FRET probe based on DNA-quantum dot conjugation

Anal Sci. 2008 Feb;24(2):181-3. doi: 10.2116/analsci.24.181.

Abstract

We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • Endonucleases / chemistry*
  • Fluorescence Resonance Energy Transfer*
  • Fluorescent Dyes / chemistry*
  • Intercalating Agents / chemistry
  • Oxazoles / chemistry*
  • Quantum Dots*
  • Sensitivity and Specificity
  • Static Electricity

Substances

  • Fluorescent Dyes
  • Intercalating Agents
  • Oxazoles
  • YoYo-3
  • DNA
  • Endonucleases