In order to develop a therapeutic control method for an artificial heart that adapts to physiological behavior for more effective medical care, we applied a resonant frequency control method to a total artificial heart, and evaluated the effectiveness through an in vitro study. The proposed control method utilizes a resonant frequency that can effectively provide blood flow. This resonant frequency was estimated using the systemic circulation model with an online system identification method in real time from two measured physiological data: aortic pressure and blood flow. The effectiveness of the proposed control method was evaluated using a mock-up circulation loop. As a result, the system gain under the resonant frequency control increased compared to the control using pulsatile frequencies that were different from the resonant frequency, when the physiological condition was changed. Therefore, we confirmed the proposed control method to be an applicable therapeutic control method that could adapt to physiological behavior for more effective medical care.