Abstract
Increased levels of interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 have been found in bronchoalveolar lavage fluid of acute respiratory distress syndrome (ARDS) patients. The authors investigated whether inflammatory-activated microvascular pulmonary endothelial cells (HMVECL) regulate expression of IL-8 and MCP-1 in the alveolar epithelial cell line A549 and studied the effects of hypoxia (5% O(2)) and hyperoxia (85% O(2)). The authors observed significant up-regulation of IL-8 and MCP-1 expression in A549 cells that was independent from the IL-1 receptor pathway but was modified by oxygen tension. These data show that the pulmonary microvascular endothelium is able to regulate epithelial cell chemokine expression in a paracrine fashion.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Hypoxia
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Cell Line, Tumor
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Chemokine CCL2 / genetics
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Chemokine CCL2 / metabolism*
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Culture Media, Conditioned / metabolism
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Endothelial Cells / metabolism*
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Epithelial Cells / metabolism*
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Humans
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Inflammation Mediators / metabolism*
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Intercellular Adhesion Molecule-1 / metabolism
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Interleukin 1 Receptor Antagonist Protein / metabolism
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Interleukin-1beta / metabolism
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Interleukin-8 / genetics
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Interleukin-8 / metabolism*
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Lung / blood supply*
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Microcirculation / metabolism
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Oxygen / metabolism
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Paracrine Communication*
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RNA, Messenger / metabolism
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Respiratory Mucosa / metabolism*
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Signal Transduction
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Time Factors
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Tumor Necrosis Factor-alpha / metabolism
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Up-Regulation
Substances
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CCL2 protein, human
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Chemokine CCL2
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Culture Media, Conditioned
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Inflammation Mediators
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Interleukin 1 Receptor Antagonist Protein
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Interleukin-1beta
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Interleukin-8
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Intercellular Adhesion Molecule-1
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Oxygen