Electrophoresis PDMS/glass chips with continuous on-chip derivatization and analysis of amino acids using naphthalene-2,3-dicarboxaldehyde as fluorogenic agent

O Yassine; P Morin; O Dispagne; L Renaud; L Denoroy; P Kleimann; K Faure; J-L Rocca; N Ouaini; R Ferrigno

doi:10.1016/j.aca.2007.12.045

Electrophoresis PDMS/glass chips with continuous on-chip derivatization and analysis of amino acids using naphthalene-2,3-dicarboxaldehyde as fluorogenic agent

Anal Chim Acta. 2008 Feb 25;609(2):215-22. doi: 10.1016/j.aca.2007.12.045. Epub 2008 Jan 10.

Authors

O Yassine¹, P Morin, O Dispagne, L Renaud, L Denoroy, P Kleimann, K Faure, J-L Rocca, N Ouaini, R Ferrigno

Affiliation

¹ Institut des Nanotechnologies de Lyon, INL, CNRS UMR5270, Université de Lyon, F-69003 Lyon, France.

PMID: 18261517
DOI: 10.1016/j.aca.2007.12.045

Abstract

In this work, we developed a PDMS electrophoresis device able to carry out on-chip derivatization and quantification of amino acids (AAs) using naphthalene-2,3-dicarboxaldehyde (NDA) as a fluorogenic agent. A chemical modification of the PDMS surface was found compulsory to achieve the derivatization of AAs with NDA and a limit of detection (LOD) of 40nM was reached for glycine. Finally, we suggested the applicability of this microdevice for the analysis of real biological samples such as a rat hippocampus microdialysate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / analysis*
Amino Acids / chemistry
Animals
Dimethylpolysiloxanes / chemistry*
Electrophoresis / instrumentation
Electrophoresis / methods*
Electrophoresis, Microchip / instrumentation
Electrophoresis, Microchip / methods*
Fluorescent Dyes / analysis*
Fluorescent Dyes / chemistry
Glass*
Hippocampus / chemistry
Hippocampus / metabolism
Naphthalenes / analysis*
Naphthalenes / chemistry
Rats

Substances

Amino Acids
Dimethylpolysiloxanes
Fluorescent Dyes
Naphthalenes
2,3-naphthalenedicarboxaldehyde