The investigation on the food selenium content in Guangzhou City of Guangdong Province showed that viscera of animal foods, aquatic products, eggs, and mutton were abundant with selenium ( > 150.0 microg x kg(-1)), followed by cereals and beans (46.5-98.3 microg x kg(-1)), vegetables (15.8 microg x kg(-1)), and fruits (4.97 microg x kg(-1)). Among vegetable foods, mushroom had the highest selenium content (46.0 microg x kg(-1)). The daily selenium intake of residents in Guangzhou was 98.5 microg, in which, 73.7% was contributed by animal foods, and 26.3% was from vegetable foods. Aquatic products, pork and rice contributed 24.8%, 22.3% and 17.2%, respectively. The food selenium content and resident daily selenium intake in Guangzhou could meet the demand of human health in the City.