Prophages in Listeria monocytogenes contain single-nucleotide polymorphisms that differentiate outbreak clones within epidemic clones

Yi Chen; Stephen J Knabel

doi:10.1128/JCM.01873-07

Prophages in Listeria monocytogenes contain single-nucleotide polymorphisms that differentiate outbreak clones within epidemic clones

J Clin Microbiol. 2008 Apr;46(4):1478-84. doi: 10.1128/JCM.01873-07. Epub 2008 Feb 6.

Authors

Yi Chen¹, Stephen J Knabel

Affiliation

¹ Center for Food Safety and Applied Nutrition, FDA, HFS-712, 5100 Paint Branch Parkway, College Park, MD 20740, USA. yi.chen@fda.hhs.gov

Abstract

A fragment end ligation-mediated PCR strategy was used to analyze the AscI pulsed-field gel electrophoresis patterns of Listeria monocytogenes epidemic clone II (ECII), which led to the identification of single-nucleotide polymorphisms (SNPs) in prophage regions that differentiated the two ECII outbreak clones. SNPs in prophages that differentiated the outbreak clones of ECIII and -IV were also identified.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Typing Techniques
Disease Outbreaks*
Humans
Listeria monocytogenes / classification*
Listeria monocytogenes / genetics
Listeria monocytogenes / virology
Listeriosis / epidemiology*
Listeriosis / microbiology
Polymorphism, Single Nucleotide*
Prophages / genetics*
Virulence