GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency panels

Katja Seme; Tina Mocilnik; Kristina Fujs Komlos; Ana Doplihar; David H Persing; Mario Poljak

doi:10.1128/JCM.01694-07

GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency panels

J Clin Microbiol. 2008 Apr;46(4):1510-3. doi: 10.1128/JCM.01694-07. Epub 2008 Feb 6.

Authors

Katja Seme¹, Tina Mocilnik, Kristina Fujs Komlos, Ana Doplihar, David H Persing, Mario Poljak

Affiliation

¹ Institute of Microbiology and Immunology, Faculty of Medicine, Zaloska 4, Ljubljana 1000, Slovenia. mario.poljak@mf.uni-lj.si.

Abstract

A prospective unblinded comparative evaluation of three assays for the detection of enteroviral RNA performed on 83 positive and 79 negative cerebrospinal fluid samples showed initial and resolved sensitivities of 90.4% and 98.8%, respectively, for the Cepheid GeneXpert enterovirus assay; 94.0% and 97.6%, respectively, for the Argene enterovirus consensus kit; and 100% and 100%, respectively, for an in-house real-time PCR. The initial and resolved specificities were 100% for all three assays.

Publication types

Evaluation Study

MeSH terms

Enterovirus / genetics
Enterovirus / isolation & purification*
Enterovirus Infections / diagnosis
Enterovirus Infections / virology
Humans
Meningitis, Viral / diagnosis*
Meningitis, Viral / virology*
RNA, Viral / cerebrospinal fluid*
Reagent Kits, Diagnostic*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity

Substances

RNA, Viral
Reagent Kits, Diagnostic