Abstract
Nanotechnology is a fundamental technology for designing and generating innovative carriers for biomacromolecular drugs. Biodegradable poly(gamma-glutamic acid)-based nanoparticles (gamma-PGA NPs) are excellent vaccine carriers capable of delivering antigenic proteins to antigen-presenting cells (APCs) and eliciting potent immune responses based on antigen-specific cytotoxic T lymphocytes. In mice, subcutaneous immunization with gamma-PGA NPs entrapping ovalbumin (OVA) more effectively inhibited the growth of OVA-transfected tumors than immunization with OVA emulsified using Freund's complete adjuvant. In addition, gamma-PGA NPs did not induce histopathologic changes after subcutaneous injection or acute toxicity through intravenous injection. Importantly, gamma-PGA NPs efficiently delivered entrapped antigenic proteins into APCs, and these antigen-capturing APCs migrated to regional lymph nodes. Our results demonstrate that a gamma-PGA NP system for antigen delivery will advance the clinical utility of vaccines against cancer.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antigen-Presenting Cells / immunology*
-
Antigens, Neoplasm / administration & dosage*
-
Antigens, Neoplasm / adverse effects
-
Antigens, Neoplasm / chemistry
-
Cancer Vaccines / administration & dosage*
-
Cancer Vaccines / adverse effects
-
Cancer Vaccines / immunology
-
Cell Line, Tumor
-
Drug Carriers
-
Freund's Adjuvant / pharmacology
-
Immunohistochemistry
-
Immunotherapy
-
Inflammation / chemically induced
-
Inflammation / pathology
-
Injections, Intravenous
-
Injections, Subcutaneous
-
Killer Cells, Natural / drug effects
-
Killer Cells, Natural / immunology
-
Mice
-
Mice, Inbred C57BL
-
Mice, Nude
-
Nanoparticles / chemistry*
-
Neoplasm Transplantation / immunology
-
Ovalbumin / immunology
-
Polyglutamic Acid / adverse effects
-
Polyglutamic Acid / analogs & derivatives*
-
Polyglutamic Acid / chemistry
-
Polyglutamic Acid / pharmacology
-
Reproducibility of Results
-
T-Lymphocytes, Cytotoxic / immunology*
Substances
-
Antigens, Neoplasm
-
Cancer Vaccines
-
Drug Carriers
-
poly(gamma-glutamic acid)
-
Polyglutamic Acid
-
Ovalbumin
-
Freund's Adjuvant