Chromosomal localization of silkworm (Bombyx mori) sericin gene 1 and chymotrypsin inhibitor 13 using fluorescence in situ hybridization

Sci China C Life Sci. 2008 Feb;51(2):133-9. doi: 10.1007/s11427-008-0025-9.

Abstract

The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5+/-1.4 position in pachytene; and that CI-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2+/-1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bombyx / enzymology
  • Bombyx / genetics*
  • Bombyx / metabolism
  • Chromosome Mapping*
  • Chromosomes / enzymology
  • Chromosomes / genetics
  • Chymotrypsin / antagonists & inhibitors*
  • Genetic Linkage
  • In Situ Hybridization, Fluorescence*
  • Insect Proteins / genetics*
  • Insect Proteins / metabolism
  • Models, Genetic
  • Sericins / genetics*
  • Sericins / metabolism*
  • Sericins / physiology
  • Serine Proteinase Inhibitors / genetics*
  • Serine Proteinase Inhibitors / metabolism
  • Serine Proteinase Inhibitors / physiology

Substances

  • Insect Proteins
  • Sericins
  • Serine Proteinase Inhibitors
  • chymotrypsin inhibitor 13 protein, Bombyx mori
  • Chymotrypsin