Analysis of cell-cell contact mediated by Ig superfamily cell adhesion molecules

Curr Protoc Cell Biol. 2001 Aug:Chapter 9:Unit 9.5. doi: 10.1002/0471143030.cb0905s11.

Abstract

This unit provides protocols to assay cell-cell adhesion and adhesive-dependent cellular functions mediated by calcium-independent adhesion molecules. These protocols have been developed for neural cell adhesion molecules of the Ig superfamily. However, most of the protocols allow a more general application to other categories of adhesion molecules and non-neural cells.

MeSH terms

  • Animals
  • Cell Adhesion Molecules / classification
  • Cell Adhesion Molecules / isolation & purification
  • Cell Adhesion Molecules / physiology*
  • Cell Adhesion*
  • Cell Line
  • Cell Line, Tumor
  • Chick Embryo
  • Chromatography, Affinity / methods*
  • Cross-Linking Reagents / pharmacology
  • Flow Cytometry / methods
  • Fluorescent Dyes
  • Genes, Immunoglobulin
  • Humans
  • Immunoblotting / methods*
  • Immunoglobulins / physiology*
  • Immunologic Capping
  • Microspheres
  • Multigene Family
  • Multiple Myeloma / pathology
  • Neurites / physiology
  • Neurites / ultrastructure
  • Recombinant Fusion Proteins / isolation & purification
  • Transfection / methods

Substances

  • Cell Adhesion Molecules
  • Cross-Linking Reagents
  • Fluorescent Dyes
  • Immunoglobulins
  • Recombinant Fusion Proteins