Abstract
We constructed a Bacillus subtilis Marburg strain that harbors deletion mutations in all seven extracytoplasmic function (ECF) sigma genes. The strain shows wild-type growth at 37 degrees C both in a complex and in a synthetic medium and exhibits wild-type sporulation. ECF sigma genes of B. subtilis are dispensable as long as no stress is imposed, although they seem to be required for quick response to stresses.
MeSH terms
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Bacillus subtilis / genetics*
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Bacillus subtilis / physiology
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Bacterial Proteins / genetics*
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Bacterial Proteins / physiology
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Cell Division / genetics
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Cell Division / physiology
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Gene Deletion
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Microbial Viability / genetics*
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Models, Biological
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Mutation
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Polymerase Chain Reaction
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Sigma Factor / genetics*
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Sigma Factor / physiology
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Spores, Bacterial / genetics
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Spores, Bacterial / physiology
Substances
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Bacterial Proteins
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Sigma Factor