S-adenosylmethionine inhibits ubiquitin-proteasome system in vitro and on rat vascular smooth muscle cells

Fabrizio D'Anselmi; Alessandra Cucina; Giuseppe Cavallaro; Mariano Bizzarri; Rosaria Adele Cavallaro; Andrea Fuso; Antonino Cavallaro; Sigfrido Scarpa

doi:10.2174/092986608783330396

S-adenosylmethionine inhibits ubiquitin-proteasome system in vitro and on rat vascular smooth muscle cells

Protein Pept Lett. 2008;15(1):58-62. doi: 10.2174/092986608783330396.

Authors

Fabrizio D'Anselmi¹, Alessandra Cucina, Giuseppe Cavallaro, Mariano Bizzarri, Rosaria Adele Cavallaro, Andrea Fuso, Antonino Cavallaro, Sigfrido Scarpa

Affiliation

¹ Department of Surgery P.Valdoni, University of Rome La Sapienza, Italy. Fabrizio.Danselmi@uniroma1.it

PMID: 18221015
DOI: 10.2174/092986608783330396

Abstract

S-adenosylmethionine is a metabolite regulating many biological processes; S-adenosylmethionine effect on ubiquitin-proteasome system (UPS) has not been studied yet. We investigated S-adenosylmethionine effects on UPS activity both in vitro, by inhibitor screening assay, and in rat vascular smooth muscle cells, by Western Blot of proteasomal targets. We found that S-adenosylmethionine inhibited UPS activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blood Vessels / drug effects
Blood Vessels / enzymology
Cells, Cultured
Muscle, Smooth, Vascular / drug effects
Muscle, Smooth, Vascular / enzymology*
Myocytes, Smooth Muscle / drug effects
Myocytes, Smooth Muscle / enzymology*
Proteasome Endopeptidase Complex / metabolism
Proteasome Inhibitors*
Rats
S-Adenosylmethionine / pharmacology*
Ubiquitin / antagonists & inhibitors*
Ubiquitin / metabolism

Substances

Proteasome Inhibitors
Ubiquitin
S-Adenosylmethionine
Proteasome Endopeptidase Complex