For the first time the influence of a number of synthetic porfirin and fluoren derivatives, coordinative germanium substances and surfactants on the biosynthesis and activity of Penicillium commune 266 alpha-L-rhamnosidase has been studied. It is shown that some of porfirin derivatives and coordinative germanium substances may be used as inducers of biosynthesis (143-430%) and also as stimulators of alpha-L-rhamnosidase activity (15-44%). It is more expedient to use biscitratgermanium acid in a complex with asparaginic acid (430%) or threonine (370%) as inducers of biosynthesis of enzyme. At the same time the use of porfirin derivatives, in particular meso-tetra(N-methyl-6-chinoline)porfirin tosilate or its manganese complex, is more efficient as stimulators (40%) of alpha-L-rhamnosidase activity. The utilization of such surfactants as tregaloso-, peptido- and rhamnolipids is not expedient. These compounds either do not influence or inhibit biosynthesis and activity of P. commune alpha-L-rhamnosidase. Only in concentration of 0.01% rhamnolipid increased the activity of enzyme by 7.5%.