Abstract
Twenty-two fragments of beta-actin and beta-actin-related protein were isolated from the acidic extracts of rat spleen tissue. beta-Actin fragments (75-90), (78-89), and (78-88), 0.01-1 microM, decreased live cell number of L929 murine tumor fibroblasts by 80-90%, with maximal cytotoxic effect of 30-40%. The fragments of (78-90) segment and the fragment of beta-actin-related protein (69-77) were less active (inhibitory effect up to 55%, cytotoxic-up to 25%).
Copyright (c) 2008 European Peptide Society and John Wiley & Sons, Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acids / chemistry*
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Actins / chemistry
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Actins / isolation & purification*
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Actins / pharmacology*
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Amino Acid Sequence
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Animals
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Chromatography, Gel
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Chromatography, High Pressure Liquid
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Female
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Mice
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Molecular Sequence Data
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Neoplasms / pathology*
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Peptides / chemistry
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Peptides / isolation & purification*
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Peptides / pharmacology*
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Rats
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Rats, Wistar
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Spleen / metabolism*
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Tissue Extracts / chemistry
Substances
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Acids
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Actins
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Peptides
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Tissue Extracts