beta-Actin-derived peptides isolated from acidic extract of rat spleen suppress tumor cell growth

J Pept Sci. 2008 Jul;14(7):811-8. doi: 10.1002/psc.1008.

Abstract

Twenty-two fragments of beta-actin and beta-actin-related protein were isolated from the acidic extracts of rat spleen tissue. beta-Actin fragments (75-90), (78-89), and (78-88), 0.01-1 microM, decreased live cell number of L929 murine tumor fibroblasts by 80-90%, with maximal cytotoxic effect of 30-40%. The fragments of (78-90) segment and the fragment of beta-actin-related protein (69-77) were less active (inhibitory effect up to 55%, cytotoxic-up to 25%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acids / chemistry*
  • Actins / chemistry
  • Actins / isolation & purification*
  • Actins / pharmacology*
  • Amino Acid Sequence
  • Animals
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Female
  • Mice
  • Molecular Sequence Data
  • Neoplasms / pathology*
  • Peptides / chemistry
  • Peptides / isolation & purification*
  • Peptides / pharmacology*
  • Rats
  • Rats, Wistar
  • Spleen / metabolism*
  • Tissue Extracts / chemistry

Substances

  • Acids
  • Actins
  • Peptides
  • Tissue Extracts