Fluorescence-based detection of single nucleotide permutation in DNA via catalytically templated reaction

Chem Commun (Camb). 2007 Oct 7:(37):3820-2. doi: 10.1039/b709611a.

Abstract

Templated reduction of low fluorescence azidocoumarin-PNA conjugate to high fluorescence aminocoumarin was achieved using a catalytic amount of DNA with single nucleotide resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalysis
  • Coumarins / chemistry*
  • DNA / chemistry*
  • DNA / genetics
  • Fluorescence
  • Molecular Conformation
  • Mutation
  • Peptide Nucleic Acids / chemistry*
  • Polymorphism, Single Nucleotide*
  • Time Factors

Substances

  • Coumarins
  • Peptide Nucleic Acids
  • DNA