One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

Chem Commun (Camb). 2007 Nov 7:(41):4251-3. doi: 10.1039/b708859k.

Abstract

Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Collodion / chemistry
  • Gold / chemistry*
  • Metal Nanoparticles / chemistry*
  • Nucleic Acids / chemistry*
  • Oligonucleotides / chemistry*
  • Polymerase Chain Reaction / instrumentation*
  • Polymerase Chain Reaction / methods*
  • Reagent Strips
  • Sensitivity and Specificity

Substances

  • Nucleic Acids
  • Oligonucleotides
  • Reagent Strips
  • Gold
  • Collodion