Gas chromatographic separation of fatty acid methyl esters on weakly polar capillary columns

Kouhei Yamamoto; Akemi Kinoshita; Akira Shibahara

doi:10.1016/j.chroma.2007.12.071

Gas chromatographic separation of fatty acid methyl esters on weakly polar capillary columns

J Chromatogr A. 2008 Feb 22;1182(1):132-5. doi: 10.1016/j.chroma.2007.12.071. Epub 2008 Jan 6.

Authors

Kouhei Yamamoto¹, Akemi Kinoshita, Akira Shibahara

Affiliation

¹ Department of Clinical Nutrition, Osaka Prefecture University, 7-30 Habikino 3-chome, Habikino 583-8555, Japan.

PMID: 18207151
DOI: 10.1016/j.chroma.2007.12.071

Abstract

It was found that weakly polar columns, routinely used in capillary GC for analyzing sterols, food additives, etc., can also be used for separating fatty acid methyl esters (FAMEs). On these columns, FAMEs elute in the order of their unsaturation. The equivalent chain-length value of methyl 22:6 is below 23.00. This means FAMEs within a carbon chain length, having up to six double bonds, elute before the next (one carbon longer) saturated FAME elutes. Peak identification is easy. Weakly polar columns are compatible in both GC and GC/MS systems.

MeSH terms

Chromatography, Gas / instrumentation
Chromatography, Gas / methods*
Esters
Fatty Acids / chemistry*
Fatty Acids / isolation & purification*
Gas Chromatography-Mass Spectrometry

Substances

Esters
Fatty Acids