Tooth enamel laser irradiated under certain conditions previously has been shown to have reduced subsurface demineralizations rate. The teeth were cut vertically in half with a thin diamond disc. One half of each tooth served as a control and the other half was irradiated using the CO2 laser. Laser energy was applied at incisive labial surface (15 W, 0.5 s, spot size 3.5 mm). After irradiation the specimens were coated with wax leaving only the windows 3 x 3 mm on the labial surfaces. After experimental demineralisation with 0.2 M sodium acetate and 0.1 M lactic acid solution for 10 days, dissolved calcium and phosphorus were analyzed in the demineralizating solutions using atomic absorption spectrophotometry and colorimetric method (method described by Lowey), respectively. Ca and P concentrations in demineralizing solutions per unit surface area were lower then the controls.