Inhibition of gene expression directed by small interfering RNAs in infectious bronchitis virus

H Meng; F Y Cui; X H Chen; Y C Pan

Inhibition of gene expression directed by small interfering RNAs in infectious bronchitis virus

Acta Virol. 2007;51(4):265-9.

Authors

H Meng¹, F Y Cui, X H Chen, Y C Pan

Affiliation

¹ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, PR China.

PMID: 18197734

Abstract

In this study, fourteen small interfering RNAs (siRNAs) were synthesized for targeting of coding gene sequences, specific 5'-leader sequence of mRNA and 3'-end sequence of the Infectious bronchitis virus (IBV) genome. The expression of viral genes in Vero-E6 cells was detected by real-time PCR. Obtained results indicated that the majority of siRNAs could effectively inhibit the expression of viral genes. The inhibition effect of siRNAs significantly differed among various genes and sites on the virus genome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions / genetics
5' Untranslated Regions / genetics
Animals
Chlorocebus aethiops
Gene Expression*
Genome, Viral
Infectious bronchitis virus / genetics
Infectious bronchitis virus / growth & development*
RNA, Small Interfering / genetics*
RNA, Viral / biosynthesis
RNA, Viral / genetics
Reverse Transcriptase Polymerase Chain Reaction
Vero Cells

Substances

3' Untranslated Regions
5' Untranslated Regions
RNA, Small Interfering
RNA, Viral