Inactivation of mitochondrial respiratory chain complex I leads mitochondrial nitric oxide synthase to become pro-oxidative

Biochem Biophys Res Commun. 2008 Mar 21;367(4):761-7. doi: 10.1016/j.bbrc.2008.01.015. Epub 2008 Jan 11.

Abstract

We recently demonstrated that mitochondrial nitric oxide synthase (mtNOS) functionally couples with mitochondrial respiratory chain complex I to produce nitric oxide [M.S. Parihar, R.R. Nazarewicz, E. Kincaid, U. Bringold, P. Ghafourifar, Association of mitochondrial nitric oxide synthase activity with respiratory chain complex I, Biochem. Biophys. Res. Commun. 366 (2008) 23-28]. The present report shows that inactivation of complex I leads mtNOS to become pro-oxidative. Our findings suggest a crucial role for mtNOS in oxidative stress caused by mitochondrial complex I inactivation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Respiration / physiology
  • Cells, Cultured
  • Electron Transport Complex I / metabolism*
  • Mitochondria / metabolism*
  • Nitric Oxide Synthase / metabolism*
  • Oxidation-Reduction
  • Oxidative Stress / physiology*
  • Oxygen / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Reactive Oxygen Species / metabolism*
  • Superoxides / metabolism*

Substances

  • Reactive Oxygen Species
  • Superoxides
  • Nitric Oxide Synthase
  • Electron Transport Complex I
  • Oxygen