Abstract
In order to study the occurrence and co-infection of different species of Campylobacter, enteric Helicobacter and Anaerobiospirillum in dogs and cats and define a possible association between these microrganisms and gastrointestinal disorders, 190 dogs and 84 cats, either healthy or with diarrhea, were sampled between 2002 and 2003. Thirty-three C. upsaliensis, 17 C. jejuni, 2 C. helveticus, 1 C. lari isolates from dogs and 14 C. helveticus, 7 C. jejuni, 6 C. upsaliensis isolates from cats were identified using species-specific PCR and phenotypic tests. Whole cell protein profile analysis, phenotypic tests, PCR-RFLP of gyrB and a phylogenetic study of partial groEL and 16S rRNA sequences were used to identify 37 H. bilis, 22 H. canis and 14 H. cinaedi in dogs and 12 H. canis, 5 H. bilis and 2 H. cinaedi in cats. Whole cell protein profile analysis, phenotypic tests and species-specific PCR of 16S rRNA were used to identify 14 A. succiniciproducens, 12 A. thomasii isolates and one unidentified Anaerobiospirillum sp. isolate in dogs and 3 A. thomasii isolates in cats. Fifty-two animals (19%) were positive for the isolation of more than one genus. No significant statistical correlation was found between any isolates of Campylobacter, Helicobacter or Anaerobiospirillum spp. or the various co-infection rates, and the presence of diarrhea in either dogs or cats. Campylobacter isolates were also tested for antibiotic resistance using the agar dilution method.
MeSH terms
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Anaerobiospirillum / classification
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Anaerobiospirillum / drug effects
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Anaerobiospirillum / isolation & purification
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Animals
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Anti-Bacterial Agents / therapeutic use
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Campylobacter / classification
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Campylobacter / drug effects
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Campylobacter / isolation & purification
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Campylobacter Infections / diagnosis
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Campylobacter Infections / drug therapy
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Campylobacter Infections / microbiology
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Campylobacter Infections / veterinary
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Cat Diseases / diagnosis
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Cat Diseases / drug therapy
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Cat Diseases / microbiology*
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Cats / microbiology*
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Diarrhea / diagnosis
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Diarrhea / drug therapy
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Diarrhea / microbiology
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Diarrhea / veterinary*
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Dog Diseases / diagnosis
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Dog Diseases / drug therapy
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Dog Diseases / microbiology*
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Dogs / microbiology*
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Dose-Response Relationship, Drug
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Drug Resistance, Bacterial
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Gram-Negative Bacterial Infections / diagnosis
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Gram-Negative Bacterial Infections / drug therapy
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Gram-Negative Bacterial Infections / microbiology
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Gram-Negative Bacterial Infections / veterinary*
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Helicobacter / classification
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Helicobacter / drug effects
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Helicobacter / isolation & purification
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Helicobacter Infections / diagnosis
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Helicobacter Infections / drug therapy
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Helicobacter Infections / microbiology
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Helicobacter Infections / veterinary
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Microbial Sensitivity Tests / veterinary
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / methods
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Polymerase Chain Reaction / veterinary
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Prevalence
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RNA, Ribosomal, 16S / genetics
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Species Specificity
Substances
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Anti-Bacterial Agents
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RNA, Ribosomal, 16S
Associated data
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GENBANK/EF428122
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GENBANK/EF569185
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GENBANK/EU144017
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GENBANK/EU144018
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GENBANK/EU144019
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GENBANK/EU233438
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GENBANK/EU233439
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GENBANK/EU233440
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GENBANK/EU233441
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GENBANK/EU233442
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GENBANK/EU233443
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GENBANK/EU233444
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GENBANK/EU233445
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GENBANK/EU233446
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GENBANK/EU233447
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GENBANK/EU233448
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GENBANK/EU233449
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GENBANK/EU233450
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GENBANK/EU233451
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GENBANK/EU233452
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GENBANK/EU233453
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GENBANK/EU233454