Novel multiplex PCR assay for simultaneous identification of community-associated methicillin-resistant Staphylococcus aureus strains USA300 and USA400 and detection of mecA and Panton-Valentine leukocidin genes, with discrimination of Staphylococcus aureus from coagulase-negative staphylococci

J Clin Microbiol. 2008 Mar;46(3):1118-22. doi: 10.1128/JCM.01309-07. Epub 2007 Dec 26.

Abstract

We developed a novel multiplex PCR assay for rapid identification and discrimination of the USA300 and USA400 strains and concomitant detection of Panton-Valentine leukocidin genes, with simultaneous discrimination of methicillin-resistant Staphylococcus aureus strains from methicillin-susceptible S. aureus strains, S. aureus strains from coagulase-negative staphylococci, and staphylococci from other bacteria.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Toxins / genetics*
  • Bacterial Typing Techniques
  • Coagulase / metabolism*
  • Community-Acquired Infections / microbiology*
  • Electrophoresis, Gel, Pulsed-Field
  • Exotoxins / genetics*
  • Humans
  • Leukocidins / genetics*
  • Methicillin / pharmacology
  • Methicillin Resistance / genetics*
  • Microbial Sensitivity Tests
  • Polymerase Chain Reaction / methods*
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / enzymology
  • Staphylococcus aureus / genetics

Substances

  • Anti-Bacterial Agents
  • Bacterial Toxins
  • Coagulase
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • Methicillin