To assess the influence of preferences on food and nutritional intake in a group of adolescent high-level athletes, 22 male soccer players (14-16 years) were recruited. Individuals were asked to fill in a specific questionnaire including 15 food groups that had to be ranked according to their preferences. Three categories were established: "Like" (ranked 1-5), "Indifferent" (6-10), and "Dislike" (11-15). Dietary intake was assessed using the weighed food method (for nutrient intake) and a quantitative open-ended food frequency questionnaire (for the number of standard portions of each food group ingested daily). The main preferences were Meat, poultry and derivates (ranked 1-5 in 83% of individuals) and Pasta (58%), while Vegetables (ranked 11-15 in 82%) and Fish (64%) were the main dislikes. The most frequently consumed food groups were Fruits and fruit juices (3.9 portions/day), Bread (3.0), and Biscuits, confectionery and sweets (3.0). No statistical differences were found in food consumption between preference groups, and no relation was found between preferences and nutritional intake, except for those individuals who especially like Bread, which had statistically higher energy and carbohydrate intake. Food preferences and food and nutritional intake of adolescent high-level soccer players were, effectively, unrelated.