Synthesis of an anthrose derivative and production of polyclonal antibodies for the detection of anthrax spores

Sandrine G Y Dhénin; Vincent Moreau; Nathalie Morel; Marie-Claire Nevers; Hervé Volland; Christophe Créminon; Florence Djedaïni-Pilard

doi:10.1016/j.carres.2007.11.030

Synthesis of an anthrose derivative and production of polyclonal antibodies for the detection of anthrax spores

Carbohydr Res. 2008 Aug 11;343(12):2101-10. doi: 10.1016/j.carres.2007.11.030. Epub 2007 Dec 8.

Authors

Sandrine G Y Dhénin¹, Vincent Moreau, Nathalie Morel, Marie-Claire Nevers, Hervé Volland, Christophe Créminon, Florence Djedaïni-Pilard

Affiliation

¹ Laboratoire des Glucides UMR-CNRS 6219, Institut de Chimie de Picardie, Université de Picardie Jules Verne, 33 rue Saint-Leu, F-80039 Amiens, France.

PMID: 18155682
DOI: 10.1016/j.carres.2007.11.030

Abstract

A straightforward synthesis of a derivative of anthrose, the non-reducing terminal fragment of the antigenic tetrasaccharide from Bacillus anthracis, was achieved starting from d-galactose. This hapten is able to induce a highly specific and sensitive immune response in rabbit when attached to a carrier protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Sugars / chemical synthesis*
Animals
Antibodies, Bacterial / immunology
Bacillus anthracis / immunology*
Deoxyglucose / analogs & derivatives*
Deoxyglucose / chemical synthesis
Hemocyanins / immunology
Immunoenzyme Techniques / methods
Rabbits
Spores, Bacterial / chemistry
Spores, Bacterial / immunology*

Substances

4,6-dideoxy-4-(3-hydroxy-3-methylbutanamido)-2-O-methylglucopyranose
Amino Sugars
Antibodies, Bacterial
Hemocyanins
Deoxyglucose
keyhole-limpet hemocyanin