Abstract
Here we report the ribosomal polymerization of alpha-hydroxy acids by means of genetic code reprogramming. The flexizyme system, a ribozyme-based tRNA acylation tool, was used to re-assign individual codons to seven types of alpha-hydroxy acids, and then polyesters were synthesized under controls of the reprogrammed genetic code using a reconstituted cell-free translation system. The sequence and length of the polyester segments were specified by the mRNA template, indicating that high-fidelity ribosome expression of polyesters was possible. This work opens a door for the mRNA-directed synthesis of backbone-altered biopolymers.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
Biopolymers / biosynthesis*
-
Biopolymers / chemistry
-
Electrophoresis, Polyacrylamide Gel
-
Escherichia coli / genetics
-
Escherichia coli / metabolism
-
Genetic Code / genetics*
-
Hydroxy Acids / chemistry
-
Hydroxy Acids / metabolism
-
Oligopeptides
-
Peptides / genetics
-
Polyesters / chemistry
-
Polyesters / metabolism*
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
RNA, Transfer / genetics
-
RNA, Transfer / metabolism
-
Ribosomes / genetics
-
Ribosomes / metabolism
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
-
Biopolymers
-
Hydroxy Acids
-
Oligopeptides
-
Peptides
-
Polyesters
-
RNA, Messenger
-
RNA, Transfer
-
FLAG peptide